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Ed, and subsequently processed for anti-Myc immunoreactivity. Ten plasmid-injected embryos and ten uninjected handle embryos were documented in each group. We detected brightlylabeled cells, like a number of melanocytes, in both mitfaz25/z25 (Fig 6A and 6B) and mitfaw2/zFig 6. Forced expression of tfap2a does not rescue melanocytes in mitfa mutant zebrafish. (A-D) Nuclei of melanocytes expressing
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