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Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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The mouse tiling array (2006-07-17MM8 tiling_set38), which covers the second half of the X chromosome (ChrX:93,883,966- 165,556,020, UCSC mouse genome Feb 2006 assembly) and the complete Y chromosome, was used in this study to closely compare the H4K16 acetylation pattern between Jarid1c and Jarid1d present on the same array. Analysis was done using the manufacturer software.Supporting Information
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