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Omplex process in which large mineral (HA) crystals are formed as the organic matrix is removed, is similarly dependent on IDPs. Janet Moradian-Oldak and her collaborators John Evans and Wendy Shaw investigated the secondary structure of amelogenin and ameloblastin, two major enamel proteins. Both were shown to be IDPs [43,44], their binding partners were other IDPs (enamelin and tuftelin) and HA.
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Or dye at 37 , the fluorescence was measured at an excitation wavelength of 530 ?25 nm and an emission wavelength of 590 ?35 nm with a Bio-Tek FL600 microplate fluorescence reader. Triplicate samples were assayed, background corrected and the results were expressed as a percentage of the values from untreated control cells. Cytokine protein arrays Cytokine expression profiling was performed on cul
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Nucleation clusters. In calcium phosphate solution, the presence of amelogenin stabilized small particles, aggregates with two or three particles and larger aggregates (pre-nucleation clusters). While needle shaped particles of hydroxyapatite were eventually formed, the amorphous aggregates were stabilized by amelogenin, leading to the statement that amelogenin inhibited (retarded) mineralization
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