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The discrepancy in results. These results raise the point that earlier PET scans with these tracers to detect early pharmacodynamic changes may not fully predict the later restaging imaging CT scan results. In conclusion, inhibition of oncogenic MAPK signaling through MEK1 and MEK2 by TAK733 results in antitumor activity in vitro against a large subset of melanoma cell lines. We confirmed the prev
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Mutants (sensitive: M207; resistant: M244) and BRAF mutants (sensitive: M229 and M249; resistant: M233 and M263) were cultured with 50 nM and 500 nM of TAK733 for 48 hours and stained with DAPI for cell cycle analysis.Discussion Initial data testing MEK inhibitors in melanoma cell lines suggested a high level and selective sensitivity in BRAF V600E mutant melanoma cell lines, with low sensitivity
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G buffer, washed and then incubated with 1.5 ml of culture supernatants from either RAW, DRAW or CDRAW macrophages for 1.5 h. After washing, 1 ml ofMaterials and methodsCells RAW264.7 cells, a mouse macrophage cell line derived from an Abelson murine leukemia virus-induced tumor in BALB/c mice, were kindly donated by T. Michiels (Christian de Duve Institute of Cellular Pathology, UCL, Belgium). DR
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