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G depression in physically ill people or those with cancer [62]. Thus, an AD considered as having no specific benefit for a given condition may prove beneficial for certain specific PC patients. As a result, it is difficult for prescribing physicians to assess the potential benefit of using them in clinical practice in PC settings where several conditions are involved.Strengths and limitations of
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K plates with clear bottom (Costar) at 105 cells per well in RPMI.B for 10 to 15h prior to Bb-stimulation. Immediately preceding stimulation, the RPMI.B media was washed to remove serum and APCs were pre-loaded with 10 2', 7'dichlorodihydrofluorescein-diacetate (DCFH-DA, i.e. DCF; Invitrogen) in serum-free HBSS for 15 minutes at 37 before washing to remove the excess dye. These loaded APCs were
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For the Sry transgenic mice, R. Watkins (UCLA), R. Bijlani (UCLA), M. Cheng (UW), D. K. Nguyen (UW), N. Krakauer (UC Berkeley), and P. Mann (Tufts U) for technical and editorial assistance.Author ContributionsConceived and designed the experiments: CD JX. Performed the experiments: JX XD. Analyzed the data: CD JX XD. Wrote the paper: CD JX.Many aspects of cell and developmental biology requi
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