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(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was resolute by RT CR using Ex4-F and Ex6-R primer pair that amplifies equally Ex4a(+)WT1 and main WT1 isoforms in two various WT1-expressing cancer cells (LU99B and K562). (B-D) Actin is utilised as an inner control. Effects are representative of a few unbiased experiments.(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was determined by RT CR
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