Oocytes were exposed to toxin by applying 3?��L of toxin at 10 times its final concentration with a pipettor and manually stirring the bath for a few seconds by gently aspirating and expelling a few microlitres of the bath fluid several times with the pipettor (Wilson et?al., 2011a). All toxin exposures were conducted in a static bath to conserve toxin. Toxins were washed out by continuous perfusi
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