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Subsequent, the ratio of Ex4a(+)WT1 isoform to 17AA(+) WT1 isoform was firm by RT-PCR making use of Ex4-forward and Ex6-reverse primer pair in WT1-expressing cancer cell lines (LU99B and K562) and established to be approximately 1/2 and 1/4 in LU99B and K562 most cancers cells, respectively (Fig 4D). These benefits indicated that the Ex4a(+)WT1 isoform was expressed being a insignificant isoform t
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